| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 204303 | 25 tests | $80 | ||
| 204304 | 100 tests | $180 |
| Clone | RB6-8C5 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Mouse |
| Format | iF647 |
| Target Name | Ly-6G/Ly-6C, Gr-1 |
| Isotype | Rat IgG2b |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm. |
| Excitation Laser | Red Laser (633 nm) |
| See All Formats | Clone RB6-8C5 |
Ly6G and Ly6C are distinct but closely related cell surface proteins belonging to the Ly-6/uPAR (urokinase-type plasminogen activator receptor) superfamily. In mouse immunology, they are critical differentiation antigens used to identify and classify myeloid cells, particularly neutrophils and monocytes. While often discussed together due to historical antibody cross-reactivity, they mark different cell populations and have distinct biological roles. Structurally, both Ly6G and Ly6C are small, highly glycosylated proteins (approximately 21–25 kDa) that lack a transmembrane domain. Instead, they are tethered to the outer leaflet of the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This structure allows them to associate laterally with other membrane proteins to transduce signals, despite lacking an intracellular signaling domain of their own.
Unlike classical receptors that bind specific soluble cytokines, Ly6G and Ly6C function primarily through interactions with adhesion molecules, particularly beta-2 integrins (such as CD11b/CD18). They do not have "ligands" in the traditional sense of a hormone or growth factor; rather, they modulate cell adhesion and migration. For example, Ly6G has been shown to regulate the recruitment of neutrophils to sites of inflammation by modulating the function of surface integrins. Similarly, Ly6C is involved in the trafficking of monocytes from the bone marrow to tissues and lymph nodes, influencing their ability to adhere to the endothelium.
In mouse research, these markers are fundamental for distinguishing immune cell subsets, though they are often a source of confusion due to the "Gr-1" antigen. The widely used anti-Gr-1 antibody (clone RB6-8C5) binds to an epitope shared by both Ly6G and Ly6C. Consequently, using anti-Gr-1 depletes or stains both neutrophils (which are Ly6G-high) and monocytes (which express varying levels of Ly6C). To achieve specificity, researchers now use clone 1A8, which binds exclusively to Ly6G, allowing for the specific identification or depletion of neutrophils without affecting monocytes.
Ly6C is particularly valuable for subdividing monocyte populations into functional subsets. "Inflammatory" monocytes are characterized as Ly6C-high (and CCR2-positive), and are rapidly recruited to sites of infection where they differentiate into macrophages or dendritic cells. In contrast, "patrolling" monocytes are Ly6C-low (and CX3CR1-high), functioning to survey the vasculature and promote tissue repair. This dichotomy based on Ly6C expression has become a standard paradigm in studying murine innate immunity and inflammation.
iF647 Rat IgG2b Isotype Control Antibody
iF647 Anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody TDS
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