Anti-DYKDDDDK (1002AH1) Affinity Gel

Product Details


Clone1002AH1
ApplicationPurification, IP
ReactivityDYKDDDDK Tag (FLAG tag)
Target NameAnti-DYKDDDDK, FLAG tag
IsotypeHuman IgG1
Regulatory StatusRUO
Formulation50% Anti-DYKDDDDK (1002AH1) Affinity Gel supplied in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage&HandlingStore the vial at 4°C (DO NOT FREEZE). The unopened vial is stable for twelve months when stored at 4°C.
Recommended Usage

This cross-linked agarose resin, conjugated with anti-DYKDDDDK antibody (clone 1002AH1), is suitable for purifying DYKDDDDK-tagged recombinant proteins using either batch or column methods (with binding capacity around 1mg/mL). It can also be used for immunoprecipitation applications.

Chemical stability: Stable in 0.1 M glycine (pH 2.5–3.0), 20% ethanol, and commonly used aqueous buffers for the purification of DYKDDDDK-tagged recombinant proteins.

See All FormatsClone 1002AH1

Background Information


Anti-DYKDDDDK (1002AH1) Affinity Gel is a specialized reagent designed for the capture, immunoprecipitation (IP), and purification of FLAG-tagged proteins. The gel consists of high-affinity monoclonal antibodies immobilized on a solid support, enabling selective binding to the DYKDDDDK epitope. This allows efficient isolation of tagged proteins from complex lysates while preserving protein integrity and interactions. It is widely used in biochemical and cell biology workflows to enrich low-abundance targets and analyze protein complexes. The resin supports gentle elution conditions, making it suitable for downstream applications such as enzymatic assays, structural studies, and protein characterization.

Data Sheets


Anti-DYKDDDDK (1002AH1) Affinity Gel TDS


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