APC Anti-Human CD32 (FcγRII) Antibody

Cat # Size Price Quantity
10981125 tests$115
109812100 tests$230

Product Details


CloneIV.3
ApplicationFlow Cytometry
ReactivityHuman
FormatAPC
Target NameCD32, FcγRII, FCRII
IsotypeMouse IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserRed Laser (633 nm)
RRIDAB_3738922
Research AreasB cells, Plasma Cells, Monocytes, Macrophages, Platelets, Mast cells, Neutrophils, Autoimmunity, Infectious Diseases
See All FormatsClone IV.3

Background Information


CD32 is a ~40-kDa polymorphic transmembrane glycoprotein belonging to the immunoglobulin superfamily. Also known as FcγRII or FCRII, it is expressed on a wide variety of immune and blood cells including B cells, monocytes/macrophages, granulocytes, platelets, and it has also been detected on natural killer (NK) cells. There are multiple isoforms of CD32 (at least six produced by alternative mRNA splicing). In humans, the major functional subtypes are CD32A and CD32C (activating) and CD32B (inhibitory).

Data Sheets


APC Anti-Human CD32 (FcγRII) Antibody TDS

Related Protocols


Flow Cytometry Protocol

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Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.