| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 117001 | 25 tests | $120 | ||
| 117002 | 100 tests | $250 |
| Clone | Dacetuzumab |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Human |
| Format | APC |
| Target Name | CD40, BP50, TNFRSF5 |
| Isotype | Human IgG1 |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. APC has an excitation max at 650 nm and an emission max at 660 nm. |
| Excitation Laser | Red Laser (633 nm) |
| See All Formats | Clone Dacetuzumab |
Human CD40 is a costimulatory receptor belonging to the tumor necrosis factor receptor (TNFR) superfamily, expressed on antigen-presenting cells such as B cells, dendritic cells, and macrophages, as well as some non-immune cells. It plays a central role in immune activation by promoting B-cell proliferation, immunoglobulin class switching, and dendritic cell maturation. Structurally, CD40 is a type I transmembrane protein with an extracellular domain composed of cysteine-rich repeats, a single transmembrane region, and a cytoplasmic tail that recruits adaptor proteins (e.g., TRAFs) to initiate downstream signaling pathways.
The primary ligand for CD40 is CD40L (CD154), expressed on activated T cells. Engagement of CD40 by CD40L triggers signaling cascades such as NF-κB activation, enhancing immune responses. Dysregulation of CD40 signaling is implicated in autoimmune diseases, chronic inflammation, and cancer. In oncology, CD40 can have dual roles: promoting anti-tumor immunity through immune activation, but also, in some contexts, supporting tumor cell survival.
Dacetuzumab is a humanized monoclonal antibody targeting CD40. It binds the extracellular domain and acts as a partial agonist, stimulating CD40 signaling while also mediating antibody-dependent cellular cytotoxicity (ADCC). Clinically, dacetuzumab has been investigated בעיקר in B-cell malignancies such as non-Hodgkin lymphoma. By activating immune cells and directly targeting CD40-expressing tumor cells, it represents an immunomodulatory therapeutic strategy, although its efficacy has been variable and is often explored in combination regimens.
APC Human IgG1 Isotype Control Antibody
APC Anti-Human CD40 Antibody TDS
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