APC Anti-Mouse CD16/32 Antibody

Cat # Size Price Quantity
20180725 tests$60
201808100 tests$150

Product Details


Clone2.4G2
ApplicationFlow Cytometry
ReactivityMouse
FormatAPC
Target NameCD16/32
IsotypeRat IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. APC has an excitation max at 650 nm and an emission max at 660 nm.
Excitation LaserRed Laser (633 nm)
See All FormatsClone 2.4G2

Background Information


CD16/32 are Fc-gamma receptors (FcγRs) expressed on a variety of immune cells, including B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD16 corresponds to the low-affinity Fc receptor III (FcγRIII), while CD32 corresponds to Fc receptor II (FcγRII). These receptors bind antibody-antigen immune complexes, linking innate and adaptive immunity and mediating adaptive immune responses. In research, antibodies against CD16/CD32 are commonly used to block Fc receptor-mediated interactions, preventing non-specific binding of antibodies or immunoglobulin complexes to immune cells during experiments such as flow cytometry and immunohistochemistry, thereby improving experimental accuracy.

Isotype Control


APC Rat IgG2b Isotype Control Antibody

Data Sheets


APC Anti-Mouse CD16/32 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


APC Rat IgG2b Isotype Control Antibody


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