APC Anti-Mouse Ly-6G (Gr-1) Antibody

Product Details


Clone1A8
ApplicationFlow Cytometry
ReactivityMouse
FormatAPC
Target NameLy-6G, Lymphocyte antigen 6 complex, locus G, Gr-1
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. APC has an excitation max at 650 nm and an emission max at 660 nm.
Excitation LaserRed Laser (633 nm)
See All FormatsClone 1A8

Background Information


Lymphocyte antigen 6 complex, locus G (Ly-6G), also known as Gr-1, is a 21–25 kDa glycosylphosphatidylinositol (GPI)-anchored surface protein. In mice, it is expressed predominantly on peripheral granulocytes, especially neutrophils, and on the majority of myeloid cells in the bone marrow. Ly-6G is transiently detected on developing monocytes but is stably and highly expressed on mature neutrophils. It plays important roles in neutrophil infiltration, recruitment, and migration, and associates with β2 integrins CD11a and CD11b to regulate their expression and function. The natural ligand for Ly-6G remains unknown, and it is widely used as a specific marker for murine neutrophils in immunological studies.

Isotype Control


APC Rat IgG1 Isotype Control Antibody

Data Sheets


APC Anti-Mouse Ly-6G (Gr-1) Antibody TDS

Related Protocols


Flow Cytometry Protocol

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APC Rat IgG1 Isotype Control Antibody


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