APC/Cyanine7 Anti-Mouse Ly-6G Antibody

Cat # Size Price Quantity
20071725 tests$80
200718100 tests$240

Product Details


Clone1A8
ApplicationFlow Cytometry
ReactivityMouse
FormatAPC/Cyanine7
Target NameLy-6G, Lymphocyte antigen 6 complex, locus G
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. APC/Cyanine7 has an excitation max at 650 nm and an emission max at 774 nm.
Excitation LaserRed Laser (633 nm)
Research AreasNeutrophils, plasmacytoid Dendritic cells
See All FormatsClone 1A8

Background Information


Lymphocyte antigen 6 complex, locus G (Ly-6G), is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored surface protein. In mice, it is expressed predominantly on peripheral granulocytes, especially neutrophils, and on the majority of myeloid cells in the bone marrow. Ly-6G is transiently detected on developing monocytes but is stably and highly expressed on mature neutrophils. It plays important roles in neutrophil infiltration, recruitment, and migration, and associates with β2 integrins CD11a and CD11b to regulate their expression and function. The natural ligand for Ly-6G remains unknown, and it is widely used as a specific marker for murine neutrophils in immunological studies.

Isotype Control


APC/Cyanine7 Rat IgG2a Isotype Control Antibody

Data Sheets


APC/Cyanine7 Anti-Mouse Ly-6G Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


iF647 Anti-Mouse CD11a Antibody, Clone M17/4

PE Anti-Mouse/Human CD11b (Integrin αM) Antibody, Clone M1/70

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.