Learn about five common methods for detecting CAR expression in CAR-T cells and understand the critical difference between structural and functional detection. This educational video compares Protein L, anti-idiotype antibodies, linker/tag antibodies, qPCR/ddPCR, and fluorescent protein ligands—explaining when to use each method and why functional validation matters for CAR-T manufacturing quality control.
Topics covered: • CAR structure fundamentals • Structural vs. functional detection concepts • Five detection methods compared • Applications in CAR-T manufacturing • Choosing the right method for your needs
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