FITC Anti-Mouse CD45.2 Antibody

Product Details


Clone104
ApplicationFlow Cytometry
ReactivityMouse
FormatFITC
Target NameCD45.2, Ly-5.2, LCA
IsotypeMouse IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. FITC has an excitation max at 493 nm and an emission max at 525 nm.
RRIDAB_3739086
Research AreasNaïve T cells, B cells, terminally differentiated effector memory T cells (TEMRA) cells
See All FormatsClone 104

Background Information


CD45.2 is a mouse alloantigen variant of CD45, expressed by Ly5.2-bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45 is a 180–240 kDa receptor-type protein tyrosine phosphatase (PTP) expressed on all hematopoietic cells except mature erythrocytes and platelets. CD45.2 plays a crucial role in immune cell signaling, lymphocyte development, and activation.

Isotype Control


FITC Mouse IgG2a Isotype Control Antibody

Data Sheets


FITC Anti-Mouse CD45.2 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


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FITC Anti-Human CD45 Antibody, Clone HI30

Biotin Anti-Human CD45 Antibody, Clone HI30

Anti-Mouse CD45 Antibody, Clone 30-F11

iF647 Anti-Mouse CD45 Antibody, Clone 30-F11

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.