| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 203203 | 25 tests | $30 | ||
| 203204 | 100 tests | $80 |
| Clone | 53-7.3 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Mouse |
| Format | FITC |
| Target Name | CD5, Ly-12, Ly-1, Lyt-1, Tp67 |
| Isotype | Rat IgG2a |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. FITC has an excitation max at 493 nm and an emission max at 525 nm. |
| Excitation Laser | Blue Laser (488 nm) |
| See All Formats | Clone 53-7.3 |
CD5 is a single-chain transmembrane glycoprotein expressed predominantly on mouse T cells and B1 cells, a specialized subset of IgM-secreting B cells. It functions as a negative regulator of antigen receptor signaling by attenuating signals from the T cell receptor (TCR) and B cell receptor (BCR), thereby modulating lymphocyte activation thresholds. Through interactions with intracellular phosphatases such as SHP-1, CD5 helps maintain immune tolerance and limits autoreactive responses. CD5-expressing cells are also associated with regulatory functions, including cytokine production such as IL-10. Owing to its regulatory role, CD5 is an important marker and functional modulator in mouse T and B cell development, activation, and immune homeostasis.
FITC Rat IgG2a Isotype Control Antibody
FITC Anti-Mouse CD5 Antibody TDS
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