This whiteboard video introduces beginners to a systematic framework for multicolor flow cytometry panel design, emphasizing alignment of the biological question, instrument configuration, and fluorophore properties. Learn the step-bystep workflow—from defining biological objectives and ranking markers to matching fluorophore brightness, minimizing spectral overlap, and implementing proper controls—to generate reproducible, high-quality data.
Download the Panel Design Cheat Sheet
to guide your next experiment.
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