How is a fluorescence labeled recombinant protein different from a labeled antibody for the same target?
A labeled protein (e.g., a fluorescent ligand or receptor domain) binds its natural counter-receptor or binding partner directly, making it useful for functional binding assays, competition/blocking studies, and receptor occupancy measurements — whereas an antibody typically binds a specific epitope regardless of the protein's natural binding activity. Choose the labeled protein format when you need to assess biologically relevant binding interactions rather than simple target detection.
Can these proteins be used in flow cytometry, and are they compatible with other panel reagents?
Yes, fluorescence labeled proteins are commonly used in flow cytometry panels to detect receptor expression or ligand binding on live cells, and are designed to be compatible with standard antibody panels. As with any multicolor panel, confirm spectral compatibility and run proper compensation controls when combining labeled proteins with labeled antibodies.
What controls should I use with fluorescence labeled recombinant proteins?
Recommended controls include an unlabeled/unconjugated version of the same protein (for competition/blocking controls), an irrelevant labeled protein of the same conjugate as a background control, and standard unstained/FMO controls for gating. Species- or format-matched controls are noted on the product page where available.
What applications are these proteins validated for?
Common applications include receptor binding assays, flow cytometry-based ligand-receptor studies, ELISA-based detection, and blocking/competition assays to study receptor occupancy or antagonist activity. Specific validated applications and recommended usage concentrations vary by product and are detailed on the individual product page.
Have a product or application question? Consult our FAQs or contact us.
