iF488 Anti-Mouse CD45.2 Antibody

Product Details


Clone104
ApplicationFlow Cytometry
ReactivityMouse
FormatiF488
Target NameCD45.2, Ly-5.2, LCA
IsotypeMouse IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF488 has an excitation max at 491 nm and an emission max at 516 nm.
See All FormatsClone 104

Background Information


CD45.2 is a mouse alloantigen variant of CD45, expressed by Ly5.2-bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45 is a 180–240 kDa receptor-type protein tyrosine phosphatase (PTP) expressed on all hematopoietic cells except mature erythrocytes and platelets. CD45.2 plays a crucial role in immune cell signaling, lymphocyte development, and activation.

Isotype Control


iF488 Mouse IgG2a Isotype Control Antibody

Data Sheets


iF488 Anti-Mouse CD45.2 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


iF488 Mouse IgG2a Isotype Control Antibody


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