iF647 Anti-Biotin Antibody

Product Details


CloneM33
ApplicationFlow Cytometry
ReactivityBiotin
FormatiF647
Target NameBiotin
IsotypeMouse IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2 mg/mL
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
See All FormatsClone M33

Background Information


The recombinant anti-biotin monoclonal antibody (clone M33) specifically binds to biotin. It is suitable for use in ELISA, Western blot (WB), flow cytometry (Flow), immunoprecipitation (IP), immunohistochemistry on paraffin-embedded sections (IHC-P), and immunohistochemistry on frozen sections (IHC-F).

Data Sheets


iF647 Anti-Biotin Antibody TDS

Related Protocols


Flow Cytometry Protocol


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