iF647 Anti-Human TCR Vb13.1 Antibody

Cat # Size Price Quantity
10630525 tests$105
106306100 tests$275

Product Details


CloneH131
ApplicationFlow Cytometry
ReactivityHuman
FormatiF647
Target NameTCR Vb13.1, T cell receptor Vβ13.1 chain, TCRBV13.1, TCRBV13
IsotypeMouse IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage and HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
RRIDAB_3738715
Research AreasT cells, T cell Activation, Adaptive Immunity
See All FormatsClone H131

Background Information


TCR Vβ13.1 is a variant of the TCR β chain. The receptor is complexed with the TCR α chain and belongs to the immunoglobulin superfamily. It is expressed on a subset of T cells and some T cell clones. Variability in the β chain is generated by Vβ, Dβ, and Jβ gene rearrangement, while variability in the α chain is generated by Vα and Jα rearrangement. TCR Vβ13.1 has been shown to be related to antigen recognition and inflammation as well as diseases, such as HIV and multiple sclerosis.

Isotype Control


iF647 Mouse IgG2b Isotype Control Antibody

Data Sheets


iF647 Anti-Human TCR Vb13.1 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-Human TCR Vb5.2/5.3 Antibody, Clone MH3-2

Anti-Mouse TCR β chain Antibody, Clone H57-597-M2a

iF488 Anti-Mouse TCR β chain Antibody, Clone H57-597-M2a

APC Anti-Human TCR Vb5.2/5.3 Antibody, Clone MH3-2

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.