| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 111503 | 25 tests | $130 | ||
| 111504 | 100 tests | $270 |
| Clone | TBX21M1 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Human, Mouse |
| Format | iF647 |
| Target Name | T-bet, T box 21, T-box expressed in T cells |
| Isotype | Mouse IgG1 |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm. |
| Excitation Laser | Red Laser (633 nm) |
| See All Formats | Clone TBX21M1 |
T-bet (TBX21) is a T-box transcription factor that plays a central role in the differentiation and function of Th1 CD4⁺ T cells in both humans and mice. Its expression is induced by IFN-γ and it directly regulates the transcription of IFN-γ, IL-12 receptor β, and IL-2 through modulation of chromatin accessibility. Loss of T-bet impairs Th1 lineage commitment, while ectopic expression promotes Th1 polarization. Beyond T cells, T-bet also contributes to B cell class-switch recombination. Due to its conserved function across species, T-bet is a key regulator of cell-mediated immunity and inflammatory responses.
iF647 Mouse IgG1 Isotype Control Antibody
iF647 Anti-human/mouse T-bet Antibody TDS
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