iF647 Anti-Mouse CD16/32 Antibody

Cat # Size Price Quantity
20180325 tests$90
201804100 tests$185

Product Details


Clone2.4G2
ApplicationFlow Cytometry
ReactivityMouse
FormatiF647
Target NameCD16/32
IsotypeRat IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
RRIDAB_3739064
Research AreasNK cells, Monocytes, Macrophages, Neutrophils, ADCC, Cancer Marker, Infectious Diseases, Inflammation
See All FormatsClone 2.4G2

Background Information


CD16/32 are Fc-gamma receptors (FcγRs) expressed on a variety of immune cells, including B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD16 corresponds to the low-affinity Fc receptor III (FcγRIII), while CD32 corresponds to Fc receptor II (FcγRII). These receptors bind antibody-antigen immune complexes, linking innate and adaptive immunity and mediating adaptive immune responses. In research, antibodies against CD16/CD32 are commonly used to block Fc receptor-mediated interactions, preventing non-specific binding of antibodies or immunoglobulin complexes to immune cells during experiments such as flow cytometry and immunohistochemistry, thereby improving experimental accuracy.

Isotype Control


iF647 Rat IgG2b Isotype Control Antibody

Data Sheets


iF647 Anti-Mouse CD16/32 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-Human CD16 (FcγRIII) Antibody, Clone 016AB

iF488 Anti-Human CD16 (FcγRIII) Antibody, Clone 016AB

iF647 Anti-Human CD16 (FcγRIII) Antibody, Clone 3G8

PE Anti-Human CD16 (FcγRIII) Antibody, Clone 3G8

In Vivo Star Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2-hg1

In Vivo Star Anti-Human CD16 (FcγRIII) Antibody, Clone 3G8-hg1SL

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.