iF647 Anti-Mouse CD5 Antibody

Cat # Size Price Quantity
20320725 tests$80
203208100 tests$180

Product Details


Clone53-7.3
ApplicationFlow Cytometry
ReactivityMouse
FormatiF647
Target NameCD5, Ly-12, Ly-1, Lyt-1, Tp67
IsotypeRat IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
Research AreasT cells, T cell Activation, Adaptive Immunity
See All FormatsClone 53-7.3

Background Information


CD5 is a single-chain transmembrane glycoprotein expressed predominantly on mouse T cells and B1 cells, a specialized subset of IgM-secreting B cells. It functions as a negative regulator of antigen receptor signaling by attenuating signals from the T cell receptor (TCR) and B cell receptor (BCR), thereby modulating lymphocyte activation thresholds. Through interactions with intracellular phosphatases such as SHP-1, CD5 helps maintain immune tolerance and limits autoreactive responses. CD5-expressing cells are also associated with regulatory functions, including cytokine production such as IL-10. Owing to its regulatory role, CD5 is an important marker and functional modulator in mouse T and B cell development, activation, and immune homeostasis.

Isotype Control


iF647 Rat IgG2a Isotype Control Antibody

Data Sheets


iF647 Anti-Mouse CD5 Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


In Vivo Star Anti-Mouse TCR beta Antibody, Clone H57-597-m2a

PB Anti-Mouse TCR β chain Antibody, Clone H57-597-R1

iF647 Anti-Mouse CD19 Antibody, Clone 1D3

Frequently Asked Questions


How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.

Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.

What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.

Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.