| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 204807 | 25 µg | $85 | ||
| 204808 | 100 µg | $195 |
| Clone | GL-1 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Mouse |
| Format | iF647 |
| Target Name | CD86, B7-2, Ly-58, B70 |
| Isotype | Rat IgG2a |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use less than 0.5 µg of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm. |
| Excitation Laser | Red Laser (633 nm) |
| See All Formats | Clone GL-1 |
Mouse CD86 (also known as B7-2) is a co-stimulatory surface protein expressed primarily on antigen-presenting cells (APCs), including dendritic cells, macrophages, and B cells. It plays a central role in T cell activation by providing the necessary second signal required for full T cell priming, complementing antigen recognition through the T cell receptor. Upon immune stimulation, CD86 expression is rapidly upregulated, enabling efficient initiation of adaptive immune responses and regulation of T cell differentiation.
Structurally, CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It contains extracellular IgV- and IgC-like domains responsible for ligand binding, a single-pass transmembrane region, and a short cytoplasmic tail lacking intrinsic enzymatic activity. Despite this, CD86 can participate in intracellular signaling through association with adaptor molecules and membrane microdomains, influencing APC activation and cytokine production.
The primary ligands of CD86 are CD28 and CTLA-4 (CD152), both expressed on T cells. Interaction with CD28 delivers a positive co-stimulatory signal that promotes T cell proliferation, cytokine secretion, and survival, while binding to CTLA-4 transmits inhibitory signals that dampen immune responses and maintain tolerance.
CD86 is implicated in autoimmune diseases, transplant rejection, and chronic inflammation due to dysregulated co-stimulation. It is also involved in tumor immune evasion by influencing T cell activation thresholds.
Therapeutically, the CD86 pathway is targeted by agents such as CTLA-4-Ig fusion proteins (e.g., abatacept) that block CD28-mediated co-stimulation, providing benefit in autoimmune diseases. Modulating CD86 activity also holds promise in cancer immunotherapy and transplant tolerance strategies.
iF647 Rat IgG2a Isotype control
iF647 Anti-mouse CD86 Antibody TDS
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