iF647 Human IgG2 Isotype Control

Cat # Size Price Quantity
30290525 μg$150

Product Details


Clone1016AH2
ApplicationFlow Cytometry
ReactivityN/A
FormatiF647
Target NameHuman IgG2 Isotype Control
IsotypeHuman IgG2
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2mg/mL
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
See All FormatsClone 1016AH2

Background Information


Human IgG2 (Immunoglobulin G subclass 2) is one of the four subclasses of IgG antibodies found in human blood and extracellular fluid. It is produced by B cells and makes up about 20–25% of total IgG in human serum. IgG2 is formed by two identical heavy chains and two identical light chains

Data Sheets


iF647 Human IgG2 Isotype Control TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.