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In Vivo Star Rat IgG1 Isotype Control Antibody

Cat # Size Price Quantity
5707011 mg$150
5707025 mg$350
57070325 mg$900

  Bulk/Custom

Product Details

CloneIVSR1
ApplicationAn isotype-matched negative control for Rat IgG1, kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
ReactivityN/A
FormatLiquid
Target NameRat IgG1 Isotype Control Antibody
IsotypeRat IgG1, κ
Antibody TypeRecombinant
Regulatory StatusRUO
Formulation0.2 μM filtered solution of 1x PBS.
Endotoxin LevelLess than 1 EU/mg of protein as determined by LAL method.
See All FormatsClone IVSR1

Background Information

Rat IgG isotype control antibodies are non-specific immunoglobulins used as negative controls in immunological and cell-based assays. They are matched to the species, immunoglobulin class or subclass, and, when applicable, light-chain type of the primary antibody being tested. Rat IgG antibodies can be classified into several isotypes based on their heavy-chain composition, including IgG1, IgG2a, IgG2b, and IgG2c, depending on the rat strain and nomenclature used. Their light chains are primarily kappa (κ) or lambda (λ).


Rat IgG antibodies are glycoproteins with a typical molecular weight of approximately 150 kDa and consist of two identical heavy chains and two identical light chains. The four chains are connected by disulfide bonds, forming the characteristic Y-shaped antibody structure. The two antigen-binding sites are located within the Fab regions at the ends of the antibody arms, while the Fc region is responsible for interactions with Fc receptors and other immune components. For an isotype control, the antibody is selected to have the same or closely matched isotype and structural characteristics as the test antibody but lacks specific binding to the target antigen.


Rat IgG isotype controls are widely used in flow cytometry, immunohistochemistry, immunofluorescence, and other antibody-based assays to help evaluate nonspecific antibody binding and background staining. An appropriate isotype control should ideally match the primary antibody in species, isotype, conjugate, concentration, and antibody-to-protein ratio. By providing a measure of nonspecific interactions, rat IgG isotype controls can help researchers distinguish true antigen-specific signals from background caused by Fc-receptor interactions, cellular autofluorescence, or nonspecific antibody binding.

Data Sheets

In Vivo Star Rat IgG1 Isotype Control Antibody TDS

Frequently Asked Questions

What are In Vivo Star Biofunctional Antibodies?
In Vivo Star antibodies are premium-grade monoclonal antibodies purified and formulated specifically for use in animal studies. Each lot is manufactured under stringent quality controls to ensure the low endotoxin levels, high purity, and functional activity required for reliable in vivo results.
How are these antibodies different from standard research antibodies (e.g., for Western blot or flow cytometry)?
Standard research antibodies are optimized for in vitro detection assays and may contain preservatives, carrier proteins (like BSA), or higher endotoxin levels unsuitable for injection. In Vivo Star antibodies are formulated in injectable-grade, azide-free, low-endotoxin buffers and validated for functional bioactivity in living systems rather than just antigen binding.
What endotoxin and purity specifications do these antibodies meet?
All In Vivo Star antibodies are tested and certified to meet low-endotoxin thresholds (typically <1 EU/mg, lot-dependent) using the LAL (Limulus Amebocyte Lysate) assay, and are purified to ≥95% purity by SEC-HPLC or SDS-PAGE. Exact specifications are provided on the Certificate of Analysis (CoA) for each lot.
Are these antibodies azide-free and carrier-free?
All In Vivo Star antibodies are formulated without sodium azide and without carrier proteins such as BSA or gelatin, since both can interfere with animal physiology or immune readouts during in vivo studies.
Do you provide isotype controls?
Matched isotype control antibodies (same host species, isotype, and formulation) are available upon request for most clones to support proper experimental controls.
How should In Vivo Star antibodies be stored and handled?
Store at 4°C for short-term use and avoid repeated freeze-thaw cycles, which can reduce bioactivity; aliquoting is recommended for long-term storage per the product-specific storage instructions on the Certificate of Analysis. Always confirm storage conditions and expiration on the CoA that accompanies each lot, as these can vary slightly by antibody.

Have a product or application question? Consult our FAQs or contact us.