InnoSite™ iF700 Rabbit IgG, κ2 Isotype Control

Cat # Size Price Quantity
38000525 mg$90

Product Details


CloneMOPC-173-CB2-SH
ApplicationFlow Cytometry
ReactivityN/A
FormatiF700
Target NameRabbit IgG, K2 Isotype Control with Fc silent mutation
IsotypeRabbit IgG, Kappa2
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2 mg/mL
Storage & HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageUse at concentrations comparable to those used for the target-specific antibody. iF700 has an excitation max at 690 nm and an emission max at 710 nm.
Excitation LaserRed Laser (633 nm)
BrandInnoSite
See All FormatsClone MOPC-173-CB2-SH

Background Information


This antibody is a clone of the MOPC-173 variable region with a rabbit IgG κ2 isotype. MOPC-173 immunoglobulin has an unknown antigen specificity and is commonly used as an isotype control. It was selected based on screening across a variety of resting and activated cells, as well as live and fixed tissues from mouse, rat, and human sources. This clone carries LALA mutations to reduce or eliminate Fc-mediated effector functions.

Data Sheets


InnoSite™ iF700 Rabbit IgG, κ2 Isotype Control TDS

Related Protocols


Flow Cytometry Protocol

Frequently Asked Questions


What should I do if I don't see an isotype control available for my antibody of choice?
Contact Technical Support or request a quote for a custom conjugation of the appropriate isotype and fluorophore.

Why should I use an isotype control?
Isotype controls are antibodies with no known specificity for the target of interest, but matched in host species, isotype, and conjugate to your primary antibody. They are used to measure non-specific binding (e.g., Fc receptor binding, background staining) so that signal from your specific antibody can be distinguished from background noise.

What should I do if my isotype control shows high background binding?
High background is often observed due to a number of causes such as Fc binding, poor cell viability, spillover and unmixing error, as well as monocyte tandem binding. Read this brief tech note to learn about the causes and specific solutions to remove background signals: The Path to True Signals: Removing Background in Flow Cytometry.

Have a product or application question? Consult our FAQs or contact us.