| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 203209 | 25 µg | $120 | ||
| 203210 | 100 µg | $270 |
| Clone | 53-7.3 |
|---|---|
| Application | Flow Cytometry |
| Reactivity | Mouse |
| Format | PB |
| Target Name | CD5, Ly-12, Ly-1, Lyt-1, Tp67 |
| Isotype | Rat IgG2a |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | 0.2 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use less than 0.2 µg of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. Pacific Blue has an excitation max at 404 nm and an emission max at 455 nm. |
| Excitation Laser | Violet laser (405nm) |
| See All Formats | Clone 53-7.3 |
CD5 is a single-chain transmembrane glycoprotein expressed predominantly on mouse T cells and B1 cells, a specialized subset of IgM-secreting B cells. It functions as a negative regulator of antigen receptor signaling by attenuating signals from the T cell receptor (TCR) and B cell receptor (BCR), thereby modulating lymphocyte activation thresholds. Through interactions with intracellular phosphatases such as SHP-1, CD5 helps maintain immune tolerance and limits autoreactive responses. CD5-expressing cells are also associated with regulatory functions, including cytokine production such as IL-10. Owing to its regulatory role, CD5 is an important marker and functional modulator in mouse T and B cell development, activation, and immune homeostasis.
PB Rat IgG2a Isotype Control Antibody
PB Anti-Mouse CD5 Antibody TDS
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