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PE Anti-human CD252 (OX40L) Antibody

Cat # Size Price Quantity
11530125 tests$120
115302100 tests$275

  Bulk/Custom

Product Details

CloneOxelumab
ApplicationFlow Cytometry
ReactivityHuman
FormatPE
Target NameCD252, OX40L, Gp34, CD134L, TNFSF4
IsotypeHuman IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. PE has an excitation max at 565 nm and an emission max at 575 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
Research AreasCo-stimulation, Dendritic cells, B cells, Macrophages, T cell survival, Th1 cells, Th2 cells, Autoimmunity
See All FormatsClone Oxelumab

Background Information

Human OX40L (CD252) is a member of the tumor necrosis factor (TNF) ligand superfamily and serves as a key costimulatory molecule in adaptive immunity. It is primarily expressed on activated antigen-presenting cells, including dendritic cells, B cells, and macrophages. OX40L binds to its receptor OX40 (CD134) on activated T cells, promoting T-cell proliferation, survival, and cytokine production. Structurally, OX40L is a type II transmembrane protein with a short cytoplasmic N-terminus, a transmembrane domain, and an extracellular TNF homology domain that mediates trimerization and receptor binding.

The primary ligand interaction of OX40L is with OX40, forming a bidirectional signaling axis that enhances T-cell responses and supports the development of memory T cells. Dysregulation of the OX40–OX40L pathway is implicated in autoimmune diseases, chronic inflammation, and allergic disorders, where excessive T-cell activation contributes to pathology. Conversely, this pathway is also important in anti-tumor immunity by sustaining effector T-cell function.

Oxelumab is a humanized monoclonal antibody targeting OX40L. By binding to OX40L, oxelumab blocks its interaction with OX40, thereby inhibiting downstream T-cell activation. This mechanism makes it particularly relevant for treating inflammatory and allergic diseases such as asthma. Therapeutically, oxelumab aims to dampen overactive immune responses, although its clinical development has shown mixed outcomes. Modulation of the OX40–OX40L axis remains an important strategy in both immunosuppressive and immunostimulatory therapeutic contexts.

Isotype Control

PE Human IgG1 Isotype Control Antibody

Data Sheets

PE Anti-human CD252 (OX40L) Antibody TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.
Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.
What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.
Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.