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PE Anti-Human CD298

Cat # Size Price Quantity
10910925 tests$110
109110100 tests$250

  Bulk/Custom

Product Details

CloneLNH-94
ApplicationFlow Cytometry
ReactivityHuman
FormatPE
Target NameCD298, ATPB-3, Na, K-ATPase beta-3 polypeptide
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. PE has an excitation max at 565 nm and an emission max at 575 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
Research AreasOsmoregulation
See All FormatsClone LNH-94

Background Information

CD298, also known as the sodium/potassium-transporting ATPase subunit beta-3 or ATP1B3, is a 42 kDa type II transmembrane glycoprotein encoded by the ATP1B3 gene in humans. It is part of the Na⁺/K⁺-ATPase enzyme complex, which consists of a catalytic α subunit and a regulatory β subunit. CD298 belongs to the family of Na⁺/K⁺ and H⁺/K⁺ ATPase beta chain proteins and specifically to the Na⁺/K⁺-ATPase subfamily. This integral plasma membrane protein is essential for establishing and maintaining the electrochemical gradients of sodium and potassium ions across the cell membrane—gradients that are crucial for osmoregulation, sodium-coupled transport of various molecules, and the electrical excitability of nerve and muscle cells. The β3 subunit plays a regulatory role by assisting in the proper assembly and membrane localization of the α/β heterodimer complex. CD298 is broadly expressed across various tissues, including all leukocyte populations.

Isotype Control

PE Mouse IgG1 Isotype Control Antibody

Data Sheets

PE Anti-Human CD298 TDS

Related Protocols

Flow Cytometry Protocol

Frequently Asked Questions

How do I determine the optimal antibody concentration/titer for my experiment?
We recommend titrating each new antibody lot on your own cell type and instrument, since optimal concentration depends on cell density, target expression level, and cytometer sensitivity. Suggested starting dilutions or test sizes (e.g., µg or µl per 10^6 cells) are provided on the product page as a starting reference point, not a fixed requirement.
Can these antibodies be used for intracellular or intranuclear staining, or only surface staining?
Intended use (surface, intracellular, or both) is specified per product, indicated under Application. The appropriate Related Protocol is linked for each product. If you need to detect both surface and intracellular markers in the same panel, confirm each antibody's compatibility with your fixation/permeabilization protocol before combining them.
What controls should I use alongside these antibodies?
Matched isotype controls (same host species, isotype, and conjugate) are available for most clones to help distinguish specific binding from background/non-specific staining and are linked on each product page. Unstained, single-color compensation, and fluorescence-minus-one (FMO) controls are also recommended, especially for multicolor panel design.
Can I combine InnoCyto Flow Cytometry antibodies into a multicolor panel?
Yes — antibodies across our catalog are designed to be panel-compatible, and conjugate options are offered specifically to support multiplexing. When building a panel, pair bright fluorophores with low-expression targets, avoid excessive spectral overlap, and confirm compensation is set up correctly for your specific fluorochrome combination. Use our Panel Builder to simplify the process.

Have a product or application question? Consult our FAQs or contact us.