PE Anti-Human TCR Vb5.2/5.3 Antibody

Product Details


CloneMH3-2
ApplicationFlow Cytometry
ReactivityHuman
FormatPE
Target NameTCR Vb5.2/5.3
IsotypeMouse IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. PE has an excitation max at 565 nm and an emission max at 575 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
See All FormatsClone MH3-2

Background Information


Vβ5 T cell receptor (TCR Vβ5) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ+ T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses. Patients with multiple sclerosis present an expansion of Vβ5.2+/5.3+ autoreactive T cells.

Isotype Control


PE Mouse IgG2a Isotype Control Antibody

Data Sheets


PE Anti-Human TCR Vb5.2/5.3 Antibody TDS

Related Protocols


Flow Cytometry Protocol

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PE Mouse IgG2a Isotype Control Antibody


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