InnoCyto - PE Human CD20 Protein (TrxA tag)

PE Human CD20 Protein (TrxA tag)

Cat #	Size	Price	Quantity
801201	25 ug	$245
801202	100 ug	$595

Bulk/Custom

Product Details

Application	Flow Cytometry
Format	Liquid, PE
Expression Host	E.coli
Target Name	CD20, B1, Bp35, MS4A1
Species	Human
Sources	Recombinant Human CD20 protein with C-terminusTrxA tag is expressed in E.coli and conjugated to PE.
Accession Number	P11836
Molecular Weight	The protein has a predicted molecular weight of 54kDa. Under DTT-reducing conditions, it migrates at approximately 70 kDa on SDS-PAGE prior to conjugation.
Affinity Tag	C-TrxA
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.

Background Information

CD20 is a B cell-specific surface molecule that plays a key role in B cell activation and regulation and is best known as one of the most successful therapeutic targets in immunology and oncology. It is expressed on B cells from the late pre-B cell stage through mature and memory B cells but is absent on early pro-B cells and terminally differentiated plasma cells. This expression pattern makes CD20 an ideal marker for identifying and targeting the majority of circulating and tissue-resident B cells.

Structurally, CD20 is a small, non-glycosylated integral membrane protein with four transmembrane helices, two extracellular loops, and intracellular N- and C-terminal domains. Unlike many CD molecules, CD20 does not belong to the immunoglobulin superfamily and lacks a long cytoplasmic signaling motif. Instead, CD20 is thought to function as part of a membrane complex involved in ion transport, particularly calcium flux, which is critical for B cell activation and proliferation. Functionally, CD20 contributes to the regulation of B cell receptor (BCR) signaling by influencing calcium entry following antigen engagement. Through modulation of intracellular calcium levels, CD20 affects B cell activation, cell cycle progression, and differentiation. While CD20 is not essential for B cell development, it plays an important role in optimizing B cell responses during immune activation. A notable feature of CD20 is that it does not have a clearly defined natural ligand. Its activity appears to be mediated through homotypic interactions, association with other membrane proteins, and organization within lipid rafts rather than classical ligand–receptor binding. This lack of ligand has not limited its therapeutic utility, as CD20 is stably expressed and poorly internalized, properties that are advantageous for antibody-based targeting.

CD20 is implicated in a range of diseases characterized by pathological B cell activity. It is highly expressed on most B cell non-Hodgkin lymphomas and chronic lymphocytic leukemia, making it a valuable diagnostic marker. In autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus, autoreactive CD20⁺ B cells contribute to disease progression through autoantibody production and antigen presentation.

Therapeutically, CD20 has revolutionized the treatment of B cell-mediated diseases. Monoclonal antibodies targeting CD20 deplete B cells through mechanisms including antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity, and induction of apoptosis. CD20-targeted therapies are widely used in hematologic malignancies and autoimmune disorders and have established B cell depletion as a powerful and durable therapeutic strategy.

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PE Human CD20 Protein (TrxA tag)

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