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Recombinant Mouse IL-2 Protein

Cat # Size Price Quantity
67030120 μg$150
670302100 μg$395

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, 2 mM DTT, pH7.4
Expression HostE.coli
Target NameIL-2, T-cell growth factor (TCGF)
SpeciesMouse
accession numberP04351
SourcesA DNA sequence encoding the soluble form of mouse IL2 (P04351) (Ala 21-Gln 169) was expressed, with an initial Met at the N-terminus.
Molecular WeightThe recombinant mouse IL2 consists of 150 amino acids and has a calculated molecular mass of 17.4KDa. It migrates as an approximately 19 KDa in SDS-PAGE under reducing conditions.
Affinity TagNone
Purity> 90 % as determined by SDS-PAGE.
Regulatory StatusRUO
Endotoxin level< 1.0 EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Background Information

Mouse interleukin-2 (IL-2) is a pleiotropic cytokine that plays a central role in regulating immune responses, particularly the activation, proliferation, differentiation, and survival of T lymphocytes. It is produced primarily by activated CD4+ and CD8+ T cells following antigen recognition and T-cell receptor stimulation. IL-2 is essential for clonal expansion of antigen-specific T cells and helps coordinate adaptive immunity. It also supports the development and maintenance of regulatory T cells (Tregs), which suppress excessive immune activation and preserve immune tolerance.



Mouse IL-2 is a secreted protein belonging to the common gamma-chain cytokine family. It is synthesized as a precursor containing a signal peptide that is removed during secretion, producing a mature cytokine of approximately 133 amino acids. The mature protein adopts a compact, predominantly alpha-helical structure characteristic of this cytokine family. IL-2 signals through the IL-2 receptor (IL-2R), which consists of three subunits: IL-2Rα (CD25), IL-2Rβ (CD122), and the common gamma chain (γc or CD132). CD25 increases ligand-binding affinity, while CD122 and CD132 form the principal signaling components. Receptor engagement activates JAK1/JAK3 and downstream STAT5 signaling, promoting immune-cell responses.



Dysregulated IL-2 signaling can contribute to autoimmune and inflammatory diseases when immune regulation is impaired, while insufficient signaling may compromise Treg function and immune tolerance. IL-2 also influences antitumor immunity by supporting the expansion and activity of effector T cells and natural killer (NK) cells. However, its effects depend on dose, receptor expression, and cellular context; IL-2 can stimulate immune activation while also expanding immunosuppressive Tregs.



Therapeutically, IL-2 and engineered IL-2 variants are investigated for cancer immunotherapy and immune-mediated disorders. Strategies include enhancing effector T-cell and NK-cell activity or preferentially stimulating Tregs to treat autoimmune conditions. Mouse IL-2 is widely used in immunology research to study lymphocyte biology, cytokine signaling, immune tolerance, and preclinical therapeutic approaches.

Data Sheets

Recombinant Mouse IL-2 Protein TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

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