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Recombinant Mouse IL-3 Protein

Cat # Size Price Quantity
67090110 μg$175
67090250 μg$340

  Bulk/Custom

Product Details

ApplicationBioassay
FormatLyophilized from sterile PBS, pH 7.4.
Expression HostHEK293
Target NameIL-3, Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF)
SpeciesMouse
accession numberNP_034686.2
SourcesA DNA sequence encoding the mouse IL3 (NP_034686.2) (Met1-Cys166) was expressed.
Molecular WeightThe recombinant mouse IL3 consists of 140 amino acids and predicts a molecular mass of 15.7 KDa. The molecular weight of this protein is around 19.5 KDa verified by SEC-MALS (Routinely tested).
Affinity TagNone
Purity> 90 % as determined by SDS-PAGE.
Regulatory StatusRUO
Endotoxin level< 0.1 EU per μg protein
Protein ConcentrationLyophilized
Storage and HandlingSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Background Information

Mouse interleukin-3 (IL-3) is a hematopoietic cytokine that regulates the proliferation, survival, differentiation, and activation of blood-forming cells. It is produced primarily by activated T lymphocytes and mast cells in response to immune stimulation. IL-3 supports the development of multiple hematopoietic lineages, including granulocytes, monocytes, basophils, eosinophils, and mast cells, and helps coordinate immune and inflammatory responses.



Mouse IL-3 is a secreted glycoprotein belonging to the class I hematopoietic cytokine family. Its mature form contains approximately 140 amino acids and adopts a characteristic four-helix bundle structure. The protein contains two intramolecular disulfide bonds and potential N-linked glycosylation sites, which may influence its biochemical properties. As a soluble cytokine, IL-3 binds to specific cell-surface receptors to initiate intracellular signaling rather than functioning as a membrane-bound ligand.



The mouse IL-3 receptor consists of an IL-3-specific alpha subunit (IL-3Rα, CD123) and a signaling beta subunit. Unlike humans, mice express two beta subunits: the common beta chain (βc, CD131), which is shared with the receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-5 (IL-5), and an IL-3-specific beta chain (βIL-3). The latter can bind mouse IL-3 directly, although productive signaling requires the receptor alpha subunit. Receptor activation stimulates pathways such as JAK2/STAT5, PI3K/AKT, and MAPK, promoting cellular growth, survival, and functional activation.



Dysregulated IL-3 signaling contributes to allergic inflammation, asthma, immune-mediated disorders, and experimental models of inflammatory disease. IL-3 may also support the growth and survival of certain leukemic cells, making its receptor system relevant to hematologic cancer research. Therapeutically, IL-3 signaling is being investigated as a target for controlling excessive inflammation, while recombinant IL-3 has been studied for its ability to stimulate hematopoietic recovery after bone marrow suppression. In preclinical research, mouse IL-3 is valuable for studying cytokine biology, hematopoiesis, immune regulation, and potential cytokine-targeted therapies.

Data Sheets

Recombinant Mouse IL-3 Protein TDS

Frequently Asked Questions

What are InnoCyto's Cytokines and Growth Factors?
These are recombinantly expressed signaling proteins, including interleukins, interferons, chemokines, and growth factors, that mediate cell communication, proliferation, differentiation, and immune activation. They are produced for use as research reagents to stimulate, modulate, or study cellular responses in vitro and in vivo.
What expression systems are these proteins produced in, and does it matter for my application?
Cytokines and growth factors may be produced in mammalian, insect, or E. coli expression systems depending on the protein, and the system affects properties like glycosylation, folding, and bioactivity. Non-glycosylated proteins from E. coli are often suitable for cell culture stimulation assays, while glycosylation-dependent proteins may require mammalian- or insect-expressed versions for full biological activity — check the product page for the expression system and recommended applications.
How is bioactivity measured and confirmed for each lot?
Bioactivity is typically confirmed using a functional cell-based assay (e.g., proliferation, differentiation, or reporter assay) relevant to the specific cytokine or growth factor, and is reported as an EC50/ED50 value or specific activity (units/mg) on the Certificate of Analysis. Always reference the lot-specific CoA rather than assuming activity is identical across lots or products.
Are these proteins carrier-free, and does that matter for my experiment?
Carrier-free formulations (without BSA or other stabilizing proteins) are available for applications where carrier protein could interfere with downstream analysis, such as certain in vivo studies or proteomic work, while carrier-containing formulations offer improved stability for routine cell culture use. Choose the format that matches your assay's sensitivity to background protein content.
Can these cytokines and growth factors be used for in vivo studies?
Mouse proteins may be used in vivo, but suitability depends on your endotoxin requirements for in vivo use. Endotoxin minimum threshholds vary by product and are indicated on the individual product page. Always confirm the endotoxin specification and formulation on the Certificate of Analysis before using a product in an in vivo setting.

Have a product or application question? Consult our FAQs or contact us.