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APC Human CD32b/c (FcγRIIB) Protein (C-His)

Cat # Size Price Quantity
82070325 ug$245
820704100 ug$595

  Bulk/Custom

Product Details

ApplicationFlow Cytometry
FormatLiquid, APC
Expression HostCHO
Target NameCD32b/c, FCGR2B, C, FcRII-b, c, CD32, FCG2, IGFR9
SpeciesHuman
SourcesRecombinant CD32b/c /Fc gamma RIIb (Ala46-Pro217) with C-terminus His-tag is expressed in CHO cell and conjugated to APC.
Accession NumberP31994
Molecular WeightThe protein has a predicted molecular weight of 20.9 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity TagC-His
Regulatory StatusRUO
Formulation1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Research AreasB cells, Plasma Cells, Monocytes, Macrophages, Platelets, Mast cells, Neutrophils, Autoimmunity, Infectious Diseases

Background Information

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins belonging to the Ig superfamily of low-affinity IgG Fc receptors. CD32B contains an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail, while CD32C has an immunoreceptor tyrosine-based activation motif (ITAM). Both receptors can bind monomeric IgG and IgG complexes, and are expressed on B cells, monocytes, macrophages, granulocytes, platelets, and mast cells. CD32B primarily acts as a negative regulator, inhibiting immune cell activation, proliferation, endocytosis, phagocytosis, and degranulation. In contrast, CD32C has an activating function, contributing to immune responses. Together, they play crucial roles in regulating immune cell functions and maintaining immune homeostasis. CD32B and CD32C share almost identical extracellular domain. The aa sequence of Fc gamma RIIB and Fc gamma RIIC are the same for the region expressed (Ala46-Pro217)

Data Sheets

APC Human CD32b/c (FcγRIIB) Protein (C-His) TDS

Related Protocols

Flow Cytometry Protocol

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Frequently Asked Questions

How is a fluorescence labeled recombinant protein different from a labeled antibody for the same target?
A labeled protein (e.g., a fluorescent ligand or receptor domain) binds its natural counter-receptor or binding partner directly, making it useful for functional binding assays, competition/blocking studies, and receptor occupancy measurements — whereas an antibody typically binds a specific epitope regardless of the protein's natural binding activity. Choose the labeled protein format when you need to assess biologically relevant binding interactions rather than simple target detection.
Can these proteins be used in flow cytometry, and are they compatible with other panel reagents?
Yes, fluorescence labeled proteins are commonly used in flow cytometry panels to detect receptor expression or ligand binding on live cells, and are designed to be compatible with standard antibody panels. As with any multicolor panel, confirm spectral compatibility and run proper compensation controls when combining labeled proteins with labeled antibodies.
What controls should I use with fluorescence labeled recombinant proteins?
Recommended controls include an unlabeled/unconjugated version of the same protein (for competition/blocking controls), an irrelevant labeled protein of the same conjugate as a background control, and standard unstained/FMO controls for gating. Species- or format-matched controls are noted on the product page where available.
What applications are these proteins validated for?
Common applications include receptor binding assays, flow cytometry-based ligand-receptor studies, ELISA-based detection, and blocking/competition assays to study receptor occupancy or antagonist activity. Specific validated applications and recommended usage concentrations vary by product and are detailed on the individual product page.

Have a product or application question? Consult our FAQs or contact us.