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Human CD32b/c (FcγRIIB) Protein (C-His)

Human CD32b/c /Fc gamma RIIb (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The purity of this protein appears to be greater than 95%.

Cat #	Size	Price	Quantity
820601	25 ug	$145
820602	100 ug	$295

Bulk/Custom

Product Details

Application	ELISA, BLI
Format	Liquid, Purified
Expression Host	CHO
Target Name	CD32b/c, FCGR2B, C, FcRII-b, c, CD32, FCG2, IGFR5
Species	Human
Sources	Recombinant CD32b/c /Fc gamma RIIb (Ala46-Pro217) with C-terminus His-tag is expressed in CHO cell.
Accession Number	P31994
Molecular Weight	The protein has a predicted molecular weight of 20.9 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE.
Affinity Tag	C-His
Purity	>95% based on SDS-PAGE under reducing condition
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level	Less than 0.1 EU/µg protein as determined by the LAL method
Protein Concentration	25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research Areas	B cells, Plasma Cells, Monocytes, Macrophages, Platelets, Mast cells, Neutrophils, Autoimmunity, Infectious Diseases

Background Information

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins belonging to the Ig superfamily of low-affinity IgG Fc receptors. CD32B contains an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail, while CD32C has an immunoreceptor tyrosine-based activation motif (ITAM). Both receptors can bind monomeric IgG and IgG complexes, and are expressed on B cells, monocytes, macrophages, granulocytes, platelets, and mast cells. CD32B primarily acts as a negative regulator, inhibiting immune cell activation, proliferation, endocytosis, phagocytosis, and degranulation. In contrast, CD32C has an activating function, contributing to immune responses. Together, they play crucial roles in regulating immune cell functions and maintaining immune homeostasis. CD32B and CD32C share almost identical extracellular domain. The aa sequence of Fc gamma RIIB and Fc gamma RIIC are the same for the region expressed (Ala46-Pro217)

Frequently Asked Questions

How is a fluorescence labeled recombinant protein different from a labeled antibody for the same target?

A labeled protein (e.g., a fluorescent ligand or receptor domain) binds its natural counter-receptor or binding partner directly, making it useful for functional binding assays, competition/blocking studies, and receptor occupancy measurements — whereas an antibody typically binds a specific epitope regardless of the protein's natural binding activity. Choose the labeled protein format when you need to assess biologically relevant binding interactions rather than simple target detection.

Can these proteins be used in flow cytometry, and are they compatible with other panel reagents?

Yes, fluorescence labeled proteins are commonly used in flow cytometry panels to detect receptor expression or ligand binding on live cells, and are designed to be compatible with standard antibody panels. As with any multicolor panel, confirm spectral compatibility and run proper compensation controls when combining labeled proteins with labeled antibodies.

What controls should I use with fluorescence labeled recombinant proteins?

Recommended controls include an unlabeled/unconjugated version of the same protein (for competition/blocking controls), an irrelevant labeled protein of the same conjugate as a background control, and standard unstained/FMO controls for gating. Species- or format-matched controls are noted on the product page where available.

What applications are these proteins validated for?

Common applications include receptor binding assays, flow cytometry-based ligand-receptor studies, ELISA-based detection, and blocking/competition assays to study receptor occupancy or antagonist activity. Specific validated applications and recommended usage concentrations vary by product and are detailed on the individual product page.

Have a product or application question? Consult our FAQs or contact us.

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