Biotin Anti-His tag Antibody

Product Details


CloneBR20M03
ApplicationELISA, WB, Flow Cytometry
ReactivityHis-tag, All Species Expected
FormatBiotin
Target NameHis Tag, histidine-tag
IsotypeMouse IgG1
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration0.2 mg/mL
Storage and HandlingQuick spin the vial after receiving. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor ELISA applications, this antibody can be used at 0.5-1.0 µg/mL as the detection antibody. However, optimization by titration is suggested for best performance in each specific application.
See All FormatsClone BR20M03

Background Information


This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.

Isotype Control


Biotin Mouse IgG1 Isotype Control Antibody

Data Sheets


Biotin Anti-His tag Antibody TDS

Related Protocols


Direct ELISA Protocol

Flow Cytometry Protocol

Related Products


Mouse IgG2a Isotype Control Antibody


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