FITC Anti-His tag Antibody

Product Details


Clone1005AB
ApplicationFlow Cytometry
ReactivityHis-tag, All Species Expected
FormatFITC
Target NameHis Tag, histidine-tag
IsotypeRabbit IgG
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. FITC has an excitation max at 493 nm and an emission max at 525 nm.
Excitation LaserBlue Laser (488 nm)
See All FormatsClone 1005AB

Background Information


This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.

Isotype Control


FITC Rabbit IgG, κ2 Isotype Control Antibody

Data Sheets


FITC Anti-His tag Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


Anti-His tag Antibody, Clone BR20M03

Biotin Anti-His tag Antibody, Clone BR20M03

Anti-c-Myc Antibody, Clone 9E10

Anti-DYKDDDDK Antibody, Clone BR20M05

Anti-Human TAG-72 (Minretumomab Biosimilar), Clone Minretumomab

iF647 Anti-c-Myc Antibody, Clone 9E10

iF488 Anti-DYKDDDDK Antibody, Clone BR20M05

iF647 Anti-DYKDDDDK Antibody, Clone 1002AH1

iF488 Anti-c-Myc Antibody, Clone 1004AM2b

APC Anti-c-Myc Antibody, Clone 1004AM2b


Have a product or application question? Consult our FAQs or contact us.