Shop Now Contact

Human SECTM1 Protein (C-Fc)

Human SECTM1 Protein (C-Fc) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95% based on reducing conditions.

Cat #	Size	Price	Quantity
806001	25 ug	$145
806002	100 ug	$295

Bulk/Custom

Product Details

Application	ELISA, BLI
Format	Liquid, Purified
Expression Host	CHO
Target Name	SECTM1, K12
Species	Human
Sources	Recombinant Human SECTM1 (Gln29-Gly145) with C-terminus Fc tag is expressed in CHO cell.
Accession Number	Q8WVN6
Molecular Weight	The protein has a predicted molecular weight of 39.0 kDa. Under DTT-reducing conditions, it migrates at approximately 48 kDa on SDS-PAGE.
Affinity Tag	C-Fc
Purity	>95% based on SDS-PAGE under reducing condition
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Research Areas	T cell Activation, Co-stimulation, Monocytes, Neutrophils

Background Information

Secreted and transmembrane protein 1 (SECTM1), also known as K12, is a type I transmembrane and secreted glycoprotein of the SECTM family. It exists in both a ~27 kDa membrane-bound form and a ~20 kDa soluble form, containing two Ig-like domains and an N-linked glycosylation motif at its N-terminus. SECTM1 is primarily expressed in peripheral blood leukocytes, particularly granulocytes, and is also highly expressed in various cancer cells such as melanoma, breast cancer, and leukemia. Its expression can be significantly upregulated by IFN-γ, especially in pathological contexts like thymus disorders. SECTM1 is found in a perinuclear Golgi-like pattern, and while surface expression is often undetectable, it may rapidly be cleaved to form the soluble version. Functionally, SECTM1 is involved in hematopoietic and immune system processes and acts as a natural ligand for CD7. Through its interaction with CD7, SECTM1 promotes T cell activation, proliferation, and cytokine production, enhances monocyte migration via the PI3K pathway, and boosts NK cell activation marker expression. These roles make it a potential target for modulating immune responses in autoimmune diseases, allogeneic transplantation, and IFN-γ–related pathologies.

Frequently Asked Questions

How is a fluorescence labeled recombinant protein different from a labeled antibody for the same target?

A labeled protein (e.g., a fluorescent ligand or receptor domain) binds its natural counter-receptor or binding partner directly, making it useful for functional binding assays, competition/blocking studies, and receptor occupancy measurements — whereas an antibody typically binds a specific epitope regardless of the protein's natural binding activity. Choose the labeled protein format when you need to assess biologically relevant binding interactions rather than simple target detection.

Can these proteins be used in flow cytometry, and are they compatible with other panel reagents?

Yes, fluorescence labeled proteins are commonly used in flow cytometry panels to detect receptor expression or ligand binding on live cells, and are designed to be compatible with standard antibody panels. As with any multicolor panel, confirm spectral compatibility and run proper compensation controls when combining labeled proteins with labeled antibodies.

What controls should I use with fluorescence labeled recombinant proteins?

Recommended controls include an unlabeled/unconjugated version of the same protein (for competition/blocking controls), an irrelevant labeled protein of the same conjugate as a background control, and standard unstained/FMO controls for gating. Species- or format-matched controls are noted on the product page where available.

What applications are these proteins validated for?

Common applications include receptor binding assays, flow cytometry-based ligand-receptor studies, ELISA-based detection, and blocking/competition assays to study receptor occupancy or antagonist activity. Specific validated applications and recommended usage concentrations vary by product and are detailed on the individual product page.

Have a product or application question? Consult our FAQs or contact us.

Search Tips