iF647 Anti-His tag Antibody

Product Details


Clone1005AB
ApplicationFlow Cytometry
ReactivityHis-tag, All Species Expected
FormatiF647
Target NameHis Tag, histidine-tag
IsotypeRabbit IgG
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. iF647 has an excitation max at 656 nm and an emission max at 670 nm.
Excitation LaserRed Laser (633 nm)
See All FormatsClone 1005AB

Background Information


This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.

Isotype Control


iF647 Rabbit IgG, κ2 Isotype Control Antibody

Data Sheets


iF647 Anti-His tag Antibody TDS

Related Protocols


Flow Cytometry Protocol

Related Products


iF647 Rabbit IgG, κ2 Isotype Control Antibody


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