iF647 Anti-mouse CD28 Antibody

Product Details


Clone37.51
ApplicationFlow Cytometry
ReactivityMouse
FormatiF647
Target NameCD28, Tp44, T44
IsotypeSyrian Hamster IgG
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserRed Laser (633 nm)
See All FormatsClone 37.51

Background Information


CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the only B7 receptor constitutively expressed on naive T cells. Association of the TCR of a naive T cell with MHC antigen complex without CD28:B7 interaction results in a T cell that is anergic.

Data Sheets


iF647 Anti-mouse CD28 Antibody TDS

Related Protocols


Flow Cytometry Protocol

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