PE/iF594 Anti-Human TCR Vb5.2/5.3 antibody

Product Details


CloneMH3-2
ApplicationFlow Cytometry
ReactivityHuman
FormatPE/iF594
Target NameTCR Vb5.2/5.3
IsotypeMouse IgG2a
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 µL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. PE/iF594 has an excitation max at 565 nm and an emission max at 603 nm.
Excitation LaserBlue Laser (488 nm) Green/Yellow laser (532/561nm)
See All FormatsClone MH3-2

Background Information


Vβ5 T cell receptor (TCR Vβ5) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ+ T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses. Patients with multiple sclerosis present an expansion of Vβ5.2+/5.3+ autoreactive T cells.

Isotype Control


PE/iF594 Mouse IgG2a Isotype Control Antibody

Data Sheets


PE/iF594 Anti-Human TCR Vb5.2/5.3 antibody TDS

Related Protocols


Flow Cytometry Protocol


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