Anti-HA Tag Antibody

Product Details


Clone12CA5
ApplicationELISA, WB, Flow Cytometry
ReactivityHA tag, All Species Expected
FormatPurified
Target NameHA epitope tag, YPYDVPDYA tag, Hemagglutinin tag
IsotypeMouse IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Protein Concentration0.5 mg/mL
Storage&HandlingQuick spin the vial after receiving. The antibody solution should be stored between 2°C and 8°C without dilution.
Recommended UsageFor ELISA application, this antibody can be used at 1 ng/mL or lower as the detection antibody. For flow cytometric staining, it is recommended to use less than 0.2 ug of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
See All FormatsClone 12CA5

Background Information


The HA tag (hemagglutinin tag) is a short linear epitope (YPYDVPDYA) derived from amino acids 98–106 of influenza A hemagglutinin. With a molecular weight of approximately 1.1 kDa, it is commonly fused to the N- or C-terminus of recombinant proteins to facilitate detection, localization, and purification when protein-specific antibodies are unavailable. Anti-HA tag antibodies specifically recognize the HA epitope and are widely used in applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and intracellular flow cytometry to study protein expression, topology, and protein–protein interactions.

Isotype Control


Mouse IgG2b Isotype Control Antibody

Data Sheets


Anti-HA Tag Antibody TDS

Related Protocols


Direct ELISA Protocol

Flow Cytometry Protocol


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