Biotin Anti-His tag Antibody

Recombinant protein with His tag is coated at 50 ng_mL (5 ng_well). Biotin anti-His antibody can detect His tagged protein in the dose dependent manner. For ELISA application, this antibody can be used at 0.5-1.0 ug_mL as the detection antibody.

Cat #	Size	Price	Quantity
300603	25 ug	$90
300604	100 ug	$275

Bulk/Custom

Product Details

Clone	BR20M03
Application	ELISA, WB, Flow Cytometry
Reactivity	His-tag, All Species Expected
Format	Biotin
Target Name	His Tag, histidine-tag
Isotype	Mouse IgG1
Antibody Type	Monoclonal
Regulatory Status	RUO
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein Concentration	0.2 mg/mL
Storage and Handling	Quick spin the vial after receiving. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage	For ELISA applications, this antibody can be used at 0.5-1.0 µg/mL as the detection antibody. However, optimization by titration is suggested for best performance in each specific application.
See All Formats	Clone BR20M03

Background Information

This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.

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Biotin Anti-His tag Antibody

Product Details

Background Information

Isotype Control

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